Bacillus Cereus Mannitol Egg Yolk (MYP) Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. NCM0062 conforms to ISO and FDA / BAM formulation. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Formulated according to ISO 7932:2004, MYP was introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods and was shown to be the most effective for this purpose by Inal in 1972. B. cereus is differentiated from other members of the Bacillus group by two reactions: mannitol fermentation and lecithinase production. Mannitol fermentation on this medium produces a yellow color, due to the indicator dye, phenol red changing colour as acid is produced. B. cereus is mannitol negative and produces red colonies. The lecithinase production of B. cereus is indicated by a white precipitate around the colonies. Polymyxin is added to suppress coliforms but some Proteus spp. and Gram-positive cocci may grow.
- Analyte: bacillus cereus
- Platform: dehydrated culture media
- Formula: beef extract 1.0g/L, peptone 10.0g/L, d-mannitol 10.0g/L, sodium chloride 10.0g/L, phenol red 0.025g/L, agar 15.0g/L
- Grams per litre: 46.00
- Litres per 500g: 11.00
- Plates per 500g: 590.00
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